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Thursday, 13 , June, 2019

Easy Way for use test reagent in laboratory.

Written by jobaer , 314 Views, Date : 13 Jun, 19

Laboratory biochemistry test reagent use easy system explanation in easy way with reagent brand name and duration.

SI Test  Name Department Sample Reagent Wait R.Brand
1 SGPT(ALAT) Bio-chemistry Serum 100 µl SGPT-(R-1=800, R2=200) within 30 sec Human, Germany
2. SGOT(ASAT) Serum 100  µl SGPT-(R-1=800, R2=200) within 30 sec Human
3. S. Creatinine Serum 100  µl D.W:700(400)+NaOH:100=800 Take 500 + PIC 500= 1000 within 30 sec Human, Germany
4. Cholesterol Serum 10  µl Cholesterol 1000(1ml) wait 10 min
5. High Density lipid(HDL) Serum  µl    
6. Low  Density lipid(LDL) Serum 10  µl R1:750+ serum 10 wait 5 min . then + R2: 250  µl wait 5 min
7. Triglyceride(Tg) Serum 10 µl Tg liquid 1000(1ml) wait 10 min
8. Uria Serum 10 µl R1: 1000 + R3: 10 + Serum: 10 wait 5 min then + R2:1000
9. Blood Uria Nitrogen (BUN) Calculation
Blood Uria result ÷ 2.14 = BUN
10. HB% Serum 20 µl working reagent 5000 (5ml) 3-5 min
11. Glucose Serum 10  µl 1000  µl 10 min
12. Uric Acid(End Point) Serum 20  µl Uric acid Liquicolor: 1000(1ml) 10 min  
13. Total Protein Serum 20  µl P.Total Liquicolor: 1000(1ml) 10 min  
14. Albumin Serum 10  µl Albumin Liquicolor: 1000(1ml) 10 min  
15. Urine Protein Serum 50  µl Ultra-sensitive Protien: 3000(3ml) 10 min Biocon, Germany
16. Bilirubin Serum 100  µl working reagent(Bilirubin liquecolor): 1000(1ml) 10 min Human, Germany
17. Bilirubin(Direct) Serum 100  µl Direct Bilirubin R1: 2500 + R2: 1 drop 10 min  
18 Total Bilirubin Serum 100  µl working reagent(Total Blirubin liquicolor): 1000(1ml) + Nitrate reagent 20  µl 5 min  
19. Bilirubin(Indirect) Calculation of indirect blirubin
T.Bilirubin- D.Bilirubin = Indirect Bilirubin
20. Calcium (Ca++) Serum 20  µl Buffer solution: 500 + Color Reagent: 500, Then wait For 10 min + serum: 20 5 min
21. Lipase Procedure Same to SGPT, SGOT  
22.        

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